■ UMP3-2 UltraMicroPump (UMP3)
(two) with SYS-Micro4 Controller •Versatile injector uses microsyringes to deliver picoliter volumes •Syringes are easily installed, just snap the barrel into the clamps •Threaded shaft drives syringe plunger allowing smooth and accurate movement
Picoliter volumes
With its digital controller, UltraMicroPump III can dispense as little as 600 picoliters per incremental advance of the syringe piston (using a 0.5μL syringe). Syringes may be filled externally and then inserted into the pump or filled while mounted in the pump. Fluids injected or withdrawn are held entirely within the micro syringe to maintain a low fluid dead volume.
Positioning
For positioning, the UltraMicroPump III may be attached to any of several WPI micropositioners such as the M3301 (manual), DC3001 (motorized), or any manual stereotaxic manipulator.
An Integral component in the UMPIII system is a microprocessor-based controller, SYS-MICRO4, which provides an "intelligent" and easy-to-use interface to up to four syringe pumps. Operating parameters are set with the membrane keypad and LCD display. From the keypad the user can select the following functions: set pump to infusion or withdrawal mode, enter the volume to be infused or withdrawn, rate of delivery, and syringe type as well as synchronize the starting and stopping of any combination of syringe pumps. User parameters can be stored in the device's "non-volatile" memory for instant recall when the unit is powered on. An optional footswitch can be plugged into a connector on the rear of the controller for "hands free" start/stop operation. Computer Control - An RS-232 port on the rear of the controller can be used to connect it to a computer for use with computer control programs.
The 503207 kit is ideal for working with an UMP3 as shown below.
NORMAL MODE
| Total # of Steps | 20,000(63mm travel) |
| Minimum Dispensing Volume | 0.58nL/step (10μL syringe) |
| Linear Motion per Step | 3.175μm |
| Weight | 325g (11.5 oz.) |
| Mounting Rod Diameters | 7.9mm (0.31 in.) |
| Controller Power Requirements | 2A, 12VDC |
| Dimensions | Ø32mm x 190mm (1.3 in. x 7.5 in.) |
MICROSTEPPING MODE
Precision is increased eight fold.
The following images show various setups for microinjection. Keep in mind that parts are interchangeable. For example:
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M10 or the M9 magnetic base could be used.
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PZMIV microscope could be used instead of the PZMIII.
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M330l or the KITE micromanipulators can be used, and these micromanipulators can be placed on either side. (Keep in mind, though, If you wanted to use a KITE on the right side of the setup below, you would order a KITE-R (right hand), or if you want an M3301 on the left side, you would order an M3301-L.)
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5479 or 5052 magnetic bases are virtually interchangeable.
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One or two Nanoliters, one or two UMPIII systems, or one Nanoliter and one UMPIII may be used, as desired.
| 15867 |
Footswitch for Micro4 |
| 40500 |
RS-232 Cable, 9-pin ""D"" connector |
| 502201 |
V-clamp for Stereotaxic Frame |
| 503301 |
10' Extension cable |
| 503207 |
FrameWorks V-Base Kit - Small |
