Micropipette Holders & Half-Cells 微電極支架和半電極
WPI's
microelectrode holder-half-cells couple fluid-filled glass micropipettes
to high input impedance amplifiers. A Ag/AgCl pellet (or a silver wire)
molded into the holder body provides stable potential. Electrical
connection is made via male 2 mm pins or female 2 mm sockets. The pipette
may be mounted axially or at right angles to the holder. Pipettes are held
with screw-caps or rubber gaskets (without caps). Filling WPI
microelectrode holders with electrolytes containing chloride results in
stable electrode potential. Suitable electrolytes include KCl, NaCl and
CaCl₂.
Holders are supplied for standard WPI single capillary tubing of 1.0, 1.2,
1.5 and 2.0 mm outside diameters. (Call WPI regarding custom designs for
other glass diameters.) The holder style you select will depend on your
experimental application, space, and instrumentation.
Hints
for selecting and ordering micropipette holders
1.Determine
the required electrical connection on the holder: for example, if you wish
to connect the holder to a 2 mm pin you should select a holder equipped
with a 2 mm jack. Most WPI probes require a holder equipped with a 2 mm
jack.
2.Decide
on the required alignment of the electrical connection: either in-line
with the glass pipette, or at a right angle to it. Space considerations in
your experimental setup and requirements imposed by other pieces of
equipment typically determine which alignment is appropriate.
3.Determine
if you want to hold the glass pipette by a rubber gasket (e.g., MEH1S) or
a'screw-cap (e.g., MEH3S). Rubber gaskets offer easier insertion and
removal of glass pipettes whereas screw-caps provide more secure mounts
for micropipetters.
4.Choose
a holder with either a silver wire or a silver/silver chloride pellet for
the metal/liquid coupling. Silver/silver chloride pellets provide a more
stable low-noise baseline which is important for low-noise DC recording.
Pellets require the glass pipette and holder to be free of air bubbles to
achieve a good connection. Silver wire holders are durable and are easier
to use when the holder is equipped with a pressure port because the fluid
in the pipette does not have to be filled to the top of the pipette to
achieve a good electrical connection
5.Choose
a holder equipped with a pressure port only when you want to pressure
inject liquid from the pipette. Two types of ports are available:2.0 mm
O.D. and standard 〞syringe-style
〞luer.
The luer port is often recommended because it makes assembly and
disassembly much easier. Quick-connect luer fittings for four common sizes
of tubing(1/16 ",3/32",1/8",5/32",I.D.) are included
with each luer-equipped holder.
6.Some
non-WPI preamplifiers or headstages cannot be mounted on
micromanipulators. In such cases, a holder equipped with a rod(e.g.,
MEH8)permits the holder to be conveniently mounted on a micromanipulator.
7.Finally,
remember to specify the O.D. of the glass you will be using when you place
your order.
MEH6RF/SF is designed primarily for use with the Model 900A Micropressure System; EHB1 for use in electrode beveling; and MEH35W for microtitration of chloride with a silver wire as the electrode and a solution of silver nitrate filling the holder. MPH models do not contain Ag/AgCl half-cells and are used for pressure injection of substances through microelectrodes. PicoNozzle, used for pressure injection with PV800 Series PicoPump, includes an MPH6S holder – which may also be used to couple a micropipette to a syringe. APEH models are also designed for use in pressurized injection procedures.
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MICROELECTRODE HOLDERS
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Electric |
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Connection
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Pressure |
Screw |
Designed
for |
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Angle |
Connector |
Half-Cell |
Port |
Cap |
WPI
Products |
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APEH1
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Right
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Male
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Pellet
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No
Port |
2
Caps |
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APEH2
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Right
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Male
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Wire
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No
Port |
2
Caps |
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EHB1
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Straight
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Male
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Wire
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No
Port |
N/A
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MBS,
48000 |
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EHBF
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Straight
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Female
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Wire
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No
Port |
N/A
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MBS,
48000 |
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FOIMPH
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Straight
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Fiber
Optic |
None
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No
Port |
w/Cap
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MBS,
48000 |
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FOIMPH-LF
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Straight
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Fiber
Optic |
None
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Male
Luer |
w/Cap
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MBS,
48000 |
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MBMPH5-7
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---
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None
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None
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Female
Luer |
w/Cap
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MEH145
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45˚ |
Male
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Pellet
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No
Port |
No
Cap |
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MEH1F45
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45˚ |
Female
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Pellet
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No
Port |
No
Cap |
705,773,767,721,FD223
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MEH1R
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Right
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Male
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Pellet
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No
Port |
No
Cap |
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MEH1RF
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Right
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Female
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Pellet
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No
Port |
No
Cap |
705,773,767,721,FD223
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MEH1S
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Straight
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Male
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Pellet
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No
Port |
No
Cap |
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MEH1SF
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Straight
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Female
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Pellet
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No
Port |
No
Cap |
705,773,767,721,FD223
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MEH2R
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Right
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Male
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Pellet
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Male
Luer |
w/Cap
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MEH2RF
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Right
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Female
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Pellet
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Male
Luer |
w/Cap
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705,773,767,721,FD223
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MEH2RFW
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Right
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Female
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Wire
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Male
Luer |
w/Cap
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705,773,767,721,FD223
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MEH2RW
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Right
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Male
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Wire
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Male
Luer |
w/Cap
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MEH2S
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Straight
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Male
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Pellet
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Male
Luer |
w/Cap
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MEH2SF
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Straight
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Female
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Pellet
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Male
Luer |
w/Cap
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705,773,767,721,FD223
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MEH2SFW
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Straight
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Female
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Wire
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Male
Luer |
w/Cap
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705,773,767,721,FD223
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MEH2SW
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Straight
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Male
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Wire
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Male
Luer |
w/Cap
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MEH345
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45˚ |
Male
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Pellet
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No
Port |
w/Cap
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MEH3F45
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45˚ |
Female
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Pellet
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No
Port |
w/Cap
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705,773,767,721,FD223
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MEH3FW45
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45˚ |
Female
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Wire
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Port
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w/Cap
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MEH3R
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Right
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Male
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Pellet
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No
Port |
w/Cap
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MEH3RF
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Right
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Female
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Pellet
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No
Port |
w/Cap
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705,773,767,721,FD223
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MEH3RFW
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Right
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Female
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Wire
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No
Port |
w/Cap
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705,773,767,721,FD223
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MEH3RW
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Right
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Male
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Wire
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No
Port |
w/Cap
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MEH3S
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Straight
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Male
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Pellet
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No
Port |
w/Cap
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MEH3SB
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Straight
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Banana
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Pellet
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No
Port |
w/Cap
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ISO-80,ISO-DAM8A
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MEH3SBW
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Straight
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Banana
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Wire
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No
Port |
w/Cap
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ISO-80,ISO-DAM8A
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MEH3SF
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Straight
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Female
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Pellet
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No
Port |
w/Cap
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705,773,767,721,FD223
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MEH3SFW
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Straight
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Female
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Wire
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No
Port |
w/Cap
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705,773,767,721,FD223
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MEH3SW
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Straight
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Male
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Wire
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No
Port |
w/Cap
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MEH3W45
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45˚ |
Male
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Wire
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No
Port |
w/Cap
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705,773,767,721,FD223
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MEH6RF
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Right
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Female
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Pellet
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2.0-mm
Port |
w/Cap
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705,773,767,721,FD223
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MEH6RFW
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Right
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Female
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Wire
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2.0-mm
Port |
w/Cap
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705,773,767,721,FD223
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MEH6SF
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Straight
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Female
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Pellet
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2.0-mm
Port |
w/Cap
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705,773,767,721,FD223
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MEH6SFW
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Straight
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Female
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Wire
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2.0-mm
Port |
w/Cap
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705,773,767,721,FD223
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MEH7
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Right
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Male
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Pellet
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2.0-mm
Port |
w/Cap
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MEH7W
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Right
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Male
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Wire
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2.0-mm
Port |
w/Cap
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MEH8
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Right
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Male
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Pellet
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No
Port |
w/Cap
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MEH900R
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Right
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Male
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Pellet
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2.0-mm
Port |
w/Cap
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900A
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MEH900S
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Straight
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Male
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Pellet
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2.0-mm
Port |
w/Cap
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900A
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MPH1
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－ |
None
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None
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Female
Luer |
w/Cap
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MPH3
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－ |
None
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None
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Male
Luer |
w/Cap
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MPH4
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－ |
None
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None
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2.0-mm
Port |
w/Cap
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MPH6P
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Right
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Male
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Pellet
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Female
Luer |
w/Cap
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Piconozzle
Kit(5430-××) |
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MPH6R
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Right
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Male
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Wire
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Female
Luer |
w/Cap
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Piconozzle
Kit(5430-××) |
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MPH6S
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－ |
None
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None
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Female
Luer |
w/Cap
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Piconozzle
Kit(5430-××) |
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SPECIFY O.D. OF GLASS (-1.0, 1.2, 1.5 OR 2.0 mm-)WHEN ORDERING.
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